Abstract
Indigofera hirsuta Linn. (Leguminosae) is a well-known plant used in African traditional medicine as an anti-inflammatory, antibacterial, antiulcer, anti-venom and anticonvulsant. The air-dried aerial parts of I. hirsuta was extracted with methanol using a soxhlet extractor and fractionated into ethyl acetate (EtOAc), n-butanol (n-BuOH) and aqueous fractions (AF). The n-butanol soluble fraction when subjected to preliminary phytochemical screening revealed the presence of flavonoids, phenolic compounds, saponins and tannins. Repeated gel filtration of n-butanol soluble fraction over a Sephadex LH-20 column eluted with methanol followed by preparative thin-layer Chromatography resulted in the isolation of two flavonol glycosides (Kaempferol-3-O-β-D-glucopyranoside and Kaempferol-7-O-β-Dglucopyranoside). The structures of these compounds were elucidated using chemical tests, spectroscopic techniques (UV, FTIR, 1D and 2D-NMR), and by comparison with reference spectral data. The n-butanol soluble fraction was found to have intraperitoneal LD50 of 1264.91 mg/kg in rats. Anti-inflammatory study using carrageenan-induced paw oedema in rats indicated that, the fraction significantly (p<0.05) inhibited the paw oedema at doses of 150 and 300 mg/kg. The percentage anti-inflammatory activity at the peak hour of the highest dose tested (300 mg/kg) was higher than that of ketoprofen (10 mg/kg), the standard anti-inflammatory agent used. The result of this research suggests that the n-butanol soluble fraction of the methanolic extract of the aerial parts of Indigofera hirsuta contains compounds with anti-inflammatory activity
ABSTRACT
The Nigerian National Policy on Education (NPE) was developed to meet national technological and economic needs. One of the ways...
Background of the study
As physical and time-consuming operations are digitalized,...
Background of the Study
Delivery care preferences refer to the choices made by pregnant women regarding the type of care they receive dur...
Background of the Study
Risk-based auditing (RBA) has emerged as a modern auditing approach focusing on identifying and...
Background of the Study
Small and medium-sized enterprises (SMEs) are vital to the economic development of many nations, including Nigeri...
Background of the Study
Cervical cancer remains a significant public health issue worldwide, especially in low- and middle-income countri...
Background of the Study
Zoonotic diseases, which are infections transmitted between animals and humans, represent a sign...
Background of the Study
Financial reporting is vital for ensuring transparency and accountability in the management of loca...
Background of the study
The availability and equality of opportunities to receive financial services ar...